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During normal improvement, the neural cell adhesion molecule N-CAM changes on the cell-surface from a sialic acid-rich embryonic, or E form, to a quantity of adult, or A forms that have much less sialic acid (E-to-A conversion). To investigate the mobile and molecular mechanisms that underlie these adjustments, we have established circumstances under which E-to-A conversion happens in cultured explants of central nervous system tissues. Mouse cerebellum, chick spinal wire, and chick retina that categorical the E form of N-CAM have been dissected and cultured on collagen gels. After 3-6 d in culture, elevated proportions of A types were synthesized, as revealed by specific immunoprecipitation and immunoblotting.
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The rate of E-to-A conversion and the proportions of the totally different A types synthesized in vitro had been much like these noticed for the tissues in vivo at comparable instances. These outcomes suggest that the publish translational processing in vitro was just like that in vivo. The data were according to the chance that the increase in synthesis of A types occurred both in cells that had beforehand synthesized E types or in a definite inhabitants of cells that already synthesized A types. In pulse-chase experiments, the E form of N-CAM that was synthesized during the first day after explantation continued as E kind for several days, at occasions when newly synthesized N-CAM was predominantly in A forms.
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